PD-L1 ELISA 试剂盒
Quick Overview for PD-L1 ELISA 试剂盒 (ABIN5026969)
抗原
See all PD-L1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
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Analytical Method
- Quantitative
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灵敏度
- < 94 pg/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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CD274 (PD-L1) ELISA Kit
PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitWide Range PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PD-L1 Inhibitor Screening ELISA KitELISA, ScA 适用: 人 Colorimetric
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 AA 19-238 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 AA 19-239 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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- PD-L1 (CD274 (PD-L1))
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别名
- PD-L1
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途径
- Cancer Immune Checkpoints
抗原 See all PD-L1 ELISA试剂盒
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